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Test Code LHSV Herpes Simplex Virus (HSV), Molecular Detection, PCR, Varies


Ordering Guidance


If herpes simplex virus (HSV) is suspected in blood, order LHSVB / Herpes Simplex Virus (HSV), Molecular Detection, PCR, Blood.

 

If HSV is suspected in cerebrospinal fluid (CSF), order HSVC / Herpes Simplex Virus (HSV), Molecular Detection, PCR, Spinal Fluid.

 

If varicella-zoster virus is suspected, order LVZV / Varicella-Zoster Virus, Molecular Detection, PCR, Varies.



Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Body fluid

Sources: Pleural, peritoneal, ascites, pericardial, amniotic, or ocular

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Specimen Type: Swab

Supplies:

M4-RT (T605)

Bartels FlexTrans VTM-3 mL (T892)

Jiangsu VTM-3 mL (T891)

Sources: Genital, dermal, ocular, nasal, throat, or oral

Container/Tube: Multimicrobe media (M4-RT)

Specimen Volume: Entire collection

Collection Instructions: Place swab back into multimicrobe media (M4-RT, Bartels FlexTrans, or Jaingsu)

Additional Information: Source information should include main anatomical site of collection.

 

Specimen Type: Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate

Container/Tube: Sterile container

Specimen Volume: 1.5 mL

 

Specimen Type: Tissue

Supplies:

M4-RT (T605)

Bartels FlexTrans VTM-3 mL (T892)

Jiangsu VTM-3 mL (T891)

Sources: Brain, colon, kidney, liver, lung, etc.

Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, Bartels FlexTrans, or Jiangsu)

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue.

Additional Information: Source information should include main anatomical site of collection.

 

Specimen Type: Urine (<1-month old infant)

Container/Tube: Sterile container

Specimen Volume: 0.5 mL


Useful For

Aiding in the rapid diagnosis of herpes simplex virus (HSV) infections, including qualitative detection of HSV DNA in nonblood clinical specimens

 

This test should not be used to screen asymptomatic patients.

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reporting Name

Herpes Simplex Virus PCR

Specimen Type

Varies

Specimen Minimum Volume

Body or Ocular Fluid: 0.4 mL
Respiratory Specimen: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

HERPES SIMPLEX VIRUS (HSV)-1

Negative

 

HERPES SIMPLEX VIRUS (HSV)-2

Negative

Day(s) Performed

Monday through Saturday; Varies

Report Available

Same day/1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87529 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LHSV Herpes Simplex Virus PCR 94580-8

 

Result ID Test Result Name Result LOINC Value
SS001 Specimen Source 39111-0
34797 HSV 1, PCR 94581-6
34798 HSV 2, PCR 94582-4

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.