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Test Code CULFB Fibroblast Culture for Biochemical or Molecular Testing, Chorionic Villi/Products of Conception/Tissue

Useful For

Producing cell cultures that can be used for enzymatic or molecular genetic analysis

Method Name

Cell Culture

Reporting Name

Fibroblast Culture for Genetic Test

Specimen Type

Tissue


Ordering Guidance


This test is most useful for chorionic villus sampling, products of conception, and skin biopsy specimens. The specimen is cryopreserved for a minimum of 6 months.

 

If ordering this test to obtain DNA for testing at a different laboratory location, see Necessary Information.



Shipping Instructions


Advise Express Mail or equivalent if not on courier service



Necessary Information


1. Include patient clinical history with request. This information will be used for any testing performed on the cultured cells.

2. Indicate the tests to be performed on the fibroblast culture cells.

Note: For testing performed on fibroblasts at locations other than Mayo Clinic Laboratories (MCL), a MCL Test ID and test requisition for the requested laboratory are required. Prior to specimen collection, call Mayo Lab Inquiry at 800-533-1710 to confirm availability of send-out testing options. Courtesy DNA extraction on fibroblast cultures will not be performed for tests not in the MCL Referral catalog.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Autopsy

Supplies: Hank's Solution (T132)

Container/Tube: Sterile container with sterile Hank's balanced salt solution, Ringer's solution, or normal saline

Specimen Volume: 4-mm diameter

Collection Instructions:

1. Wash biopsy site with an antiseptic soap.

2. Thoroughly rinse area with sterile water.

3. Do not use alcohol or iodine preparations.

4. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.

 

Specimen Type: Chorionic villi

Supplies: CVS Media (RPMI) and Small Dish (T095)

Container/Tube: 15 mL tube containing 15 mL of transport media

Specimen Volume: 20-30 mg

Collection Instructions:

1. Collect specimen by the transabdominal or transcervical method.

2. Transfer the chorionic villi specimen to a Petri dish containing transport medium.

3. Using a stereomicroscope and sterile forceps, assess the quality and quantity of the villi and remove any blood clots and maternal decidua.

 

Specimen Type: Products of conception or stillbirth

Supplies: Hank's Solution (T132)

Container/Tube: Sterile container with sterile Hank's balanced salt solution, Ringer's solution, or normal saline

Specimen Volume: 1 cm(3) of placenta (including 20 mg of chorionic villi) and a 1 cm(3) biopsy specimen of muscle/fascia from the thigh

Collection Instructions: If a fetus cannot be specifically identified, collect 50 mg villus material or tissue that appears to be of fetal origin.

Additional Information: Do not send entire fetus.

 

Specimen Type: Cultured fibroblasts

Container/Tube: T-25 flask

Specimen Volume: 2 Full T-25 flasks

Specimen Stability Information: Ambient (preferred)/Refrigerated

 

Specimen Type: Skin biopsy

Supplies: Fibroblast Biopsy Transport Media (T115)

Container/Tube: Sterile container with any standard cell culture media (eg, minimal essential media, RPMI 1640). The solution should be supplemented with 1% penicillin and streptomycin. Tubes of culture media can be supplied upon request (Eagle's minimum essential medium with 1% penicillin and streptomycin).

Specimen Volume: 4-mm punch


Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue Refrigerated (preferred)
  Ambient 

Day(s) Performed

Monday through Friday

Report Available

41 to 42 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

Not Applicable

CPT Code Information

88233

88240

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CULFB Fibroblast Culture for Genetic Test 96300-9

 

Result ID Test Result Name Result LOINC Value
52327 Result Summary 50397-9
52329 Interpretation 69965-2
52328 Result 82939-0
CG770 Reason for Referral 42349-1
CG899 Specimen 31208-2
52331 Source 31208-2
52332 Method 85069-3
54625 Additional Information 48767-8
52333 Released By 18771-6

Forms

1. Biochemical Genetics Patient Information (T602)

2. Final Disposition of Fetal/Stillborn Remains (if fetal specimen is sent). Only required for products of conception or stillbirth specimen.

Testing Algorithm

This processing test is for culturing chorionic villi, products of conception, skin fibroblasts, or other biopsy specimens for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.

 

When this test is ordered, cryopreservation will be performed. For multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered. Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested.

 

To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.

 

If viable cells are not obtained within 30 days, client will be notified.

 

If Mycoplasma is detected, all pending fibroblast clinical testing will be canceled, and samples will be discarded.

Specimen Minimum Volume

See Specimen Required

Reference Values

Not applicable