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Test Code DIFFM Differential-Manual, Blood

Methodology

Manual-Microscopic Examination of Cells

Includes neutrophilic segs and bands, lymphocytes, monocytes, eosinophils, basophils, and RBC morphology.

Performing Laboratory

Aspen Valley Hospital

Specimen Requirements

Specimen must arrive within ≤24 hours of draw.

 

Container/Tube:  Lavender-top (EDTA) tube(s) - No other anticoagulant is acceptable.

Specimen:  3.5 mL of EDTA whole blood (minimum volume:  1.5 mL of EDTA whole blood /250 µL in MICROTAINER®)

Transport temperature:  Ambient

Collection Instructions:  Invert 8 times to mix blood.

Reference Values

NEUTROPHILS

0 days-1 month:  19-73%

1 month-2 years:  8-51%

2-10 years:  12-55%

10-18 years:  14-63%

≥18 years:  22-63%

NEUTROPHILS (ABSOLUTE COUNT)

0 days-1 month:  1.7-23.4 103/µL

1 month-2 years:  0.3-5.6 103/µL

≥2 years:  1.4-7.0 103/µL 

BAND NEUTROPHILS

0 days-1 month:  0-11%

≥1 month:  0-8%

LYMPHOCYTES

0 days-1 month:  8-33%

1 month-2 years:  14-69%

2-10 years:  8-46%

10-18 years:  9-33%

≥18 years:  22-36%

LYMPHOCYTES (ABSOLUTE COUNT)

0 days-1 month:  0.7-10.6 103/µL

1 month-2 years:  0.6-7.6 103/µL

2-14 years:  0.9-9.0 103/µL 

≥14 years:  0.9-4.0 103/µL 

MONOCYTES

0 days-1 month:  0-5%

1 month-10 years:  0-7%

≥10 years:  2-10%

MONOCYTES (ABSOLUTE COUNT)

0.1-1.1 103/µL 

EOSINOPHILS

0 days-1 month:  0-5%

≥1 month:  0-4%

EOSINOPHILS (ABSOLUTE COUNT)

0.0-0.4 103/µL 

BASOPHILS

≥0 days:  0-1%

BASOPHILS (ABSOLUTE COUNT)

0.0-0.1 103/µL 

RBC MORPHOLOGY

Normal

WBC (ABSOLUTE COUNT)

0 days-1 month:  9.0-32.0 103/µL
1 month-2 years:  5.0-13.0 103/µL

2-10 years:  4.0-12.0 103/µL

10-18 years:  4.0-10.5 103/µL

≥18 years:  4.2-11.0 103/µL

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

85007