Test Code DIFFM Differential-Manual, Blood
Methodology
Manual-Microscopic Examination of CellsIncludes neutrophilic segs and bands, lymphocytes, monocytes, eosinophils, basophils, and RBC morphology.
Performing Laboratory
Aspen Valley HospitalSpecimen Requirements
Specimen must arrive within ≤24 hours of draw.
Container/Tube: Lavender-top (EDTA) tube(s) - No other anticoagulant is acceptable.
Specimen: 3.5 mL of EDTA whole blood (minimum volume: 1.5 mL of EDTA whole blood /250 µL in MICROTAINER®)
Transport temperature: Ambient
Collection Instructions: Invert 8 times to mix blood.
Reference Values
NEUTROPHILS0 days-1 month: 19-73%
1 month-2 years: 8-51%
2-10 years: 12-55%
10-18 years: 14-63%
≥18 years: 22-63%
NEUTROPHILS (ABSOLUTE COUNT)
0 days-1 month: 1.7-23.4 103/µL
1 month-2 years: 0.3-5.6 103/µL
≥2 years: 1.4-7.0 103/µL
BAND NEUTROPHILS
0 days-1 month: 0-11%
≥1 month: 0-8%
LYMPHOCYTES
0 days-1 month: 8-33%
1 month-2 years: 14-69%
2-10 years: 8-46%
10-18 years: 9-33%
≥18 years: 22-36%
LYMPHOCYTES (ABSOLUTE COUNT)
0 days-1 month: 0.7-10.6 103/µL
1 month-2 years: 0.6-7.6 103/µL
2-14 years: 0.9-9.0 103/µL
≥14 years: 0.9-4.0 103/µL
MONOCYTES
0 days-1 month: 0-5%
1 month-10 years: 0-7%
≥10 years: 2-10%
MONOCYTES (ABSOLUTE COUNT)
0.1-1.1 103/µL
EOSINOPHILS
0 days-1 month: 0-5%
≥1 month: 0-4%
EOSINOPHILS (ABSOLUTE COUNT)
0.0-0.4 103/µL
BASOPHILS
≥0 days: 0-1%
BASOPHILS (ABSOLUTE COUNT)
0.0-0.1 103/µL
RBC MORPHOLOGY
Normal
WBC (ABSOLUTE COUNT)
0 days-1 month: 9.0-32.0 103/µL
1 month-2 years: 5.0-13.0 103/µL
2-10 years: 4.0-12.0 103/µL
10-18 years: 4.0-10.5 103/µL
≥18 years: 4.2-11.0 103/µL