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Test Code HOLDF Hematologic Disorders, Fluorescence In Situ Hybridization (FISH) Hold, Varies

Ordering Guidance

This test is designed to hold blood and bone marrow specimens only.


This test does not apply to chromosome analysis. If specimen is to be held for chromosome analysis, order HOLDC / Hematologic Disorders, Chromosome Hold, Varies.


Due to stability issues, test PCPDS / Plasma Cell Proliferative Disorder, High-Risk with Reflex Probes, Diagnostic FISH Evaluation, Bone Marrow cannot be added onto specimens held under this test.

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

Provide a reason for testing with each specimen and bone marrow pathology report (if available). The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

Specimen Required

Submit only 1 of the following specimens:



Specimen Type: Bone marrow


Preferred: Yellow top (ACD)

Acceptable: Green top (sodium heparin), lavender top (EDTA)

Specimen Volume: 1 to 2 mL

Collection Instructions: Invert several times to mix bone marrow.



Specimen Type: Blood


Preferred: Yellow top (ACD)

Acceptable: Green top (sodium heparin), lavender top (EDTA)

Specimen Volume: 6 mL

Collection Instructions: Invert several times to mix blood.


If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Useful For

Processing the bone marrow or peripheral blood specimen but delaying fluorescence in situ hybridization analysis while preliminary morphologic assessment is in process

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
AMLMF AML, Specified FISH Yes No
AMLPF Pediatric AML, FISH Yes No
BALAF Adult ALL (B-cell), FISH Yes No
BALMF ALL (B-cell), Specified FISH Yes No
BALPF Pediatric ALL (B-cell), FISH Yes No
BLPMF B-cell Lymphoma, Specified FISH Yes No
CILDF Cong Infantile Leukemia, Diag FISH Yes No
CILMF Cong Infantile Leukemia, Spec FISH Yes No
CLLDF CLL, Diagnostic FISH Yes No
CLLMF CLL, Specified FISH Yes No
COGBF COG, ALL (B-cell), FISH Yes No
COGTF COG, ALL (T-cell), FISH Yes No
EOSDF Chronic Eosinophilia, Diag FISH Yes No
EOSMF Chronic Eosinophilia, Spec FISH Yes No
HEMMF Hematologic Specified FISH Yes No
MDSDF MDS, Diagnostic FISH Yes No
MDSMF MDS, Specified FISH Yes No
MFCDF Myeloma Fixed Cell, High Risk, FISH Yes No
PHLDF Ph-like ALL(B-cell), Diag FISH Yes No
TALAF Adult ALL (T-cell), FISH Yes No
TALMF ALL (T-cell), Specified FISH Yes No
TALPF Pediatric ALL (T-cell), FISH Yes No
TLPDF T-cell Lymphoma B/BM, Diag FISH Yes No
TLPMF T-cell Lymphoma B/BM, Spec FISH Yes No

Testing Algorithm

This test is designed to hold the sample and delay fluorescence in situ hydribization (FISH) testing while preliminary morphologic assessment or flow cytometry testing is in process.


Hold policy: Upon sample receipt, the specimen will be held in the laboratory. FISH testing will not be performed unless the client contacts the laboratory and indicates that FISH testing is desired. The client must contact the Cytogenetics Laboratory at 800-533-1710 by 4 p.m. (Central time) no later than 4 business days (96 hours) after the specimen was collected. If no notification is received by this time, the order will be processed as "cancelled." Weekend communication can be deferred until Monday.

Method Name

Direct Preparation of Specimen

Reporting Name

Heme FISH Hold, B/BM

Specimen Type


Specimen Minimum Volume

Blood: 2 mL
Bone marrow: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)

Reference Values

Not applicable

Day(s) Performed

Monday through Sunday

Report Available

4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

Not Applicable

CPT Code Information

See individual reflex tests

LOINC Code Information

Result ID Test Result Name Result LOINC Value
51836 Result Summary 50397-9
51838 Interpretation 69965-2
CG666 Reason for Referral 42349-1
CG667 Specimen 31208-2
51839 Source 31208-2
CG791 Requested FISH Test 48767-8
51841 Method 85069-3
53433 Additional Information 48767-8
51842 Released by 18771-6