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Test Code HV1CM HIV-1 and HIV-2 Antibody Screen for Hemolyzed Specimens, Serum

Reporting Name

HIV-1/-2 Ab Screen Hemolyzed, S

Useful For

Screening cadaveric or hemolyzed serum specimens for HIV-1 and/or HIV-2 infection in non- symptomatic patients with or without risk factors for HIV infection


This test is not offered as a screening or confirmatory test for blood donor specimens.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
HIVDI HIV Ab Confirm / Differentiation, S No No

Testing Algorithm

This test begins with HIV-1/-2 antibody enzyme immunoassay (EIA). If HIV-1/-2 antibody EIA is reactive, then HIV antibody confirmation/differentiation by immunochromatographic method is performed at an additional charge.


See HIV Testing Algorithm (Fourth-Generation Screening Assay), Including Follow-up of Reactive Rapid Serologic Test Results

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Ordering Guidance

1. This test is not intended for testing symptomatic individuals (ie, diagnostic purposes). For testing hemolyzed specimens from such patients with or without risk factors for HIV infection, order HV1CD / HIV-1 and HIV-2 Antibodies for Cadaveric or Hemolyzed Specimens, Serum.

2. New York State clients: This test should not be requested for maternal/newborn HIV screening on specimens originating in New York State due to state regulatory requirements for expedited result reporting.

Additional Testing Requirements

If the initial enzyme immunoassay (EIA) result is negative and this test was ordered as a follow-up evaluation of a specimen with a reactive rapid HIV antibody test result, clients must call 800-533-1710 or 507-266-5700 to request supplemental testing for HIV antibody confirmation/differentiation by immunochromatography (HIVDI). The HIVDI / HIV-1 and HIV-2 Antibody Confirmation and Differentiation, Serum test is not FDA-approved for testing cadaveric specimens. If performed, test results will be reported with a disclaimer.

Necessary Information

Date of collection is required.

Specimen Required

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot serum into plastic vial.

Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 30 days
  Ambient  7 days
  Refrigerated  7 days

Reference Values


Day(s) Performed

Tuesday, Friday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information



86701 (if appropriate)

86702 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HV1CM HIV-1/-2 Ab Screen Hemolyzed, S 31201-7


Result ID Test Result Name Result LOINC Value
60357 HIV-1/-2 Ab Screen Hemolyzed, S 31201-7

Report Available

1 to 7 days

Method Name

Enzyme Immunoassay (EIA)-Screening Procedure


If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.