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Test Code MPHNU Methylphenidate and Metabolite, Random, Urine

Additional Testing Requirements

If urine creatinine is required or adulteration of the sample is suspected, also order ADULT / Adulterants Survey, Random, Urine. For more information, see ADULT / Adulterants Survey, Random, Urine.

Specimen Required

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube: Plastic urine container

Submission Container/Tube: Plastic, 5-mL tube

Specimen Volume: 5 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.

Additional Information:

1. No specimen substitutions.

2. STATS are not accepted for this test.

Useful For

Monitoring urine methylphenidate and ritalinic acid concentrations to assess compliance in patients

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Methylphenidate and Metabolite, U

Specimen Type


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 10 days
  Frozen  28 days

Reference Values



Cutoff concentrations:

Methylphenidate by LC-MS/MS: 10 ng/mL

Ritalinic Acid by LC-MS/MS: 50 ng/mL

Day(s) Performed

Monday, Wednesday, Friday

Report Available

2 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


G0480 (if appropriate)


LOINC Code Information

Test ID Test Order Name Order LOINC Value
MPHNU Methylphenidate and Metabolite, U In Process


Result ID Test Result Name Result LOINC Value
608882 Methylphenidate by LC-MS/MS 20548-4
608883 Ritalinic Acid by LC-MS/MS 72790-9
608884 Methylphenidate Interpretation 69050-3