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Test Code MTBT Mycobacterium tuberculosis Complex, Molecular Detection, PCR, Paraffin, Tissue

Useful For

Preferred method for rapid detection of Mycobacterium tuberculosis complex DNA in formalin-fixed, paraffin-embedded tissue specimens

 

Detection of M tuberculosis complex

 

The PCR assay is not intended for the detection of latent tuberculosis and must not be used as a substitute for tests intended for detection of latent tuberculosis such as the tuberculin skin test (TST/PPD) or an interferon gamma release assay (IGRA).

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

MTB complex PCR, FFPE

Specimen Type

Tissue, Paraffin


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycobacterium tuberculosis DNA is unlikely.

 

Preferred Paraffin-embedded tissue block:

Supplies: Tissue Block Container (T553)

Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)

Sources: Body tissue

Container/Tube: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block to be cut and returned.

 

Acceptable Paraffin-embedded tissue block:

Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)

Sources: Body tissue

Container/Tube: Sterile container for each individual cut section (scroll).

Collection Instructions: Perform microtomy and prepare five separate 10-micron sections. Each section (scroll) must be placed in a separate sterile container for submission.


Specimen Minimum Volume

See Specimen Required.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue, Paraffin Ambient (preferred)
  Refrigerated 

Reference Values

Not applicable

Day(s) Performed

Monday through Friday

Report Available

5 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87556-Mycobacterium tuberculosis, complex, molecular detection, PCR, Paraffin

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTBT MTB complex PCR, FFPE 38379-4

 

Result ID Test Result Name Result LOINC Value
SRCTB MTB Complex PCR, FFPE, Source 31208-2
TBRR MTB Complex PCR, FFPE, Result 38379-4