Sign in →

Test Code NIS Nickel, Serum

Reporting Name

Nickel, S

Useful For

Confirmation of an elevated urinary nickel concentration


This test is not useful for the investigation of nickel hypersensitivity.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Ordering Guidance

Urine is the preferred specimen type for determining nickel exposure and potential toxicity. Order NIU / Nickel, Urine or NICRU / Nickel/Creatinine Ratio, Random, Urine.


This test should not be ordered for the investigation of nickel hypersensitivity. Instead, order testing for lymphocyte proliferation to nickel.

Specimen Required

Patient Preparation: High concentrations of gadolinium and iodine are known to interfere with most metal tests. If either gadolinium- or iodine-containing contrast media has been administered, a specimen should not be collected for 96 hours.

Supplies: Metal Free Specimen Vial (T173)

Collection Container/Tube: Royal blue-top (metal-free, no additive)

Submission Container/Tube: 7-mL Mayo metal-free, screw-capped, vial

Specimen Volume: 2 mL

Collection Instructions:

1. Allow the specimen to clot for 30 minutes; then centrifuge the specimen to separate serum from the cellular fraction.

2. Remove the stopper. Carefully pour specimen into a Mayo metal-free, screw-capped vial, avoiding transfer of the cellular components of blood. Do not insert a pipet into the serum to accomplish transfer, and do not ream the specimen with a wooden stick to assist with serum transfer.

3. See Trace Metals Analysis Specimen Collection and Transport for complete instructions.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days METAL FREE
  Ambient  7 days METAL FREE
  Frozen  7 days METAL FREE

Reference Values

<2.0 ng/mL

Day(s) Performed


Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
NIS Nickel, S 5702-6


Result ID Test Result Name Result LOINC Value
8622 Nickel, S 5702-6

Report Available

1 to 7 days

Method Name

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)