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Test Code SUBIF Immunoglobulin G (IgG) Subtypes Immunofluorescence, Tissue


Ordering Guidance


If additional interpretation/analysis is needed, request PATHC / Pathology Consultation along with this test and send the corresponding renal pathology light microscopy and immunofluorescence (IF) slides (or IF images on a CD), electron microscopy images (prints or CD), and the pathology report.



Shipping Instructions


1. Advise shipping frozen specimens (unstained slides or tissue block) in Styrofoam transportation coolers filled with dry ice to ensure specimens are received at required specimen stability temperature.

2. Attach the green "Attention Pathology" address label (T498) to the outside of the transport container before putting into the courier mailer.



Necessary Information


A pathology/diagnostic report is required.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Unstained slides (unfixed)

Source: Kidney tissue

Slides: 4 Slides

Collection Instructions: 4 frozen tissue unstained positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick, centered on the slide, and submitted on dry ice.

 

Acceptable:

Specimen Type: Unfixed tissue block (O.C.T)

Source: Kidney tissue

Specimen Volume: Entire specimen

Collection Instructions:

1. Embed in O.C.T. compound.

2. Freeze specimen and ship on dry ice.

 

Acceptable:

Specimen Type: Wet tissue

Source: Kidney tissue

Supplies: Renal Biopsy Kit (T231)

Container/Tube: Renal Biopsy Kit, Zeus/Michel's

Specimen Volume: Entire specimen

Collection Instructions:

1. Collect specimens according to the instructions in Renal Biopsy Procedure for Handling Tissue for Light Microscopy, Immunofluorescent Histology, and Electron Microscopy.

2. If standard immunoglobulin and complement immunofluorescence has already been performed, submit the residual frozen tissue (must contain glomeruli) on dry ice.


Forms

1. Renal Biopsy Patient Information

2. If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.

Useful For

Determining the subclass of IgG antibody found in renal immunofluorescent panel and determining if the deposits are monoclonal or monotypic

Method Name

Direct Immunofluorescence

Reporting Name

IGG Subtypes Immunofluorescence

Specimen Type

Special

Specimen Stability Information

Specimen Type Temperature Time Special Container
Special Frozen

Day(s) Performed

Monday through Friday

Report Available

1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88346-Primary IF

88350-If additional IF

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SUBIF IGG Subtypes Immunofluorescence In Process

 

Result ID Test Result Name Result LOINC Value
BA0271 Interpretation 50595-8
BA0272 Participated in the Interpretation No LOINC Needed
BA0275 Report electronically signed by 19139-5
BA0276 Addendum 35265-8
BA0274 Gross Description 22634-0
BA0273 Material Received 22633-2
71618 Disclaimer 62364-5
71850 Case Number 80398-1

Reference Values

An interpretive report will be provided.