Test Code SUBIF Immunoglobulin G (IgG) Subtypes Immunofluorescence, Tissue
Ordering Guidance
If additional interpretation/analysis is needed, request PATHC / Pathology Consultation along with this test and send the corresponding renal pathology light microscopy and immunofluorescence (IF) slides (or IF images on a CD), electron microscopy images (prints or CD), and the pathology report.
Shipping Instructions
1. Advise shipping frozen specimens (unstained slides or tissue block) in Styrofoam transportation coolers filled with dry ice to ensure specimens are received at required specimen stability temperature.
2. Attach the green "Attention Pathology" address label (T498) to the outside of the transport container before putting into the courier mailer.
Necessary Information
A pathology/diagnostic report is required.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Unstained slides (unfixed)
Source: Kidney tissue
Slides: 4 Slides
Collection Instructions: 4 frozen tissue unstained positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick, centered on the slide, and submitted on dry ice.
Acceptable:
Specimen Type: Unfixed tissue block (O.C.T)
Source: Kidney tissue
Specimen Volume: Entire specimen
Collection Instructions:
1. Embed in O.C.T. compound.
2. Freeze specimen and ship on dry ice.
Acceptable:
Specimen Type: Wet tissue
Source: Kidney tissue
Supplies: Renal Biopsy Kit (T231)
Container/Tube: Renal Biopsy Kit, Zeus/Michel's
Specimen Volume: Entire specimen
Collection Instructions:
1. Collect specimens according to the instructions in Renal Biopsy Procedure for Handling Tissue for Light Microscopy, Immunofluorescent Histology, and Electron Microscopy.
2. If standard immunoglobulin and complement immunofluorescence has already been performed, submit the residual frozen tissue (must contain glomeruli) on dry ice.
Forms
1. Renal Biopsy Patient Information
2. If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.
Useful For
Determining the subclass of IgG antibody found in renal immunofluorescent panel and determining if the deposits are monoclonal or monotypic
Special Instructions
Method Name
Direct Immunofluorescence
Reporting Name
IGG Subtypes ImmunofluorescenceSpecimen Type
SpecialSpecimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Special | Frozen |
Day(s) Performed
Monday through Friday
Report Available
1 to 2 daysPerforming Laboratory

Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88346-Primary IF
88350-If additional IF
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
SUBIF | IGG Subtypes Immunofluorescence | In Process |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
BA0271 | Interpretation | 50595-8 |
BA0272 | Participated in the Interpretation | No LOINC Needed |
BA0275 | Report electronically signed by | 19139-5 |
BA0276 | Addendum | 35265-8 |
BA0274 | Gross Description | 22634-0 |
BA0273 | Material Received | 22633-2 |
71618 | Disclaimer | 62364-5 |
71850 | Case Number | 80398-1 |
Reference Values
An interpretive report will be provided.